Biotechnology by John E Smith

By John E Smith

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Howell CR, Stipanovic RD (1983) Can J Microbiol 29:321 46. Gray TR (1976) Symp Soc Gen Microbiol 26:327 47. Bennett JW (1983) In: Bennett JW, Ciegler A (eds) Secondary metabolism and differentiation in fungi. Marcel Dekker, New York, p 1 48. Campbell IM (1984) Adv Microb Physiol 25:1 49. Campbell IM, Doerfler DL, Bird BA, Remaley AT, Rosato LM, Davis BN (1982) In: Krumphanzl V, Sikyta B,Vanek Z (eds) Overproduction of microbial products. Academic Press, London, p 141 50. Jensen PR, Fenical W (1994) Annu Rev Microbiol 48:559 51.

Kuninaka [7] reexamined this work and revealed that 5¢-inosinic and guanylic acids, but not the 2¢- and 3¢-nucleotides, possess not only a potent flavor themselves but also potent flavor-enhancing activity in the presence of monosodium glutamate. Since only venom nuclease was known to cleave RNA to 5¢-nucleotides, they screened microorganisms for the activity and found nuclease P1 from a Penicillium strain [8]. Success of the enzymatic processes to produce the nucleotides from yeast RNA triggered the next challenge of nucleotide biosynthesis as described below.

42 43 45 47 49 50 50 52 52 54 54 54 56 56 57 57 58 58 Advances in Biochemical Engineering/ Biotechnology, Vol. 69 Managing Editor: Th. 2 T. Beppu Pharmaceuticals of Microbial Origins . . . . . . . Molecular Genetics of Secondary Metabolism . . . . . Genetic Engineering for Production of Heterologous Proteins Protein Engineering of G-CSF .

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